Common NMR experiment sets

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Szyperski group

The following experiments are recorded for NC samples

Common experiments GFT protocol Conventional protocol Fractionally 13C-labeled samples
  • 1D
  • 1D 15N T1
  • 1D 15N T2
  • 2D [15N,1H]-HSQC
  • 2D [15N,1H]-HSQC with wide spectral width in 15N (optional)
  • 2D [13C,1H]-HSQC aliphatic
  • 2D [13C,1H]-HSQC aromatic (optional)
  • 2D [13C,1H]-CT-HSQC aliphatic
  • 2D [13C,1H]-CT-HSQC aromatic
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 3D HN(CA)CO (optional)
  • 3D (H)CCH-TOCSY
  • 2D HE(CECDCG)CB (optional)
  • 3D 15N/13Cali/13Caro-resolved [1H,1H]-NOESY
  • (3,2)D GFT HNNCO or 3D HNCO
  • (4,3)D GFT CABCA(CO)NH
  • (4,3)D GFT HNCABCA
  • (4,3)D GFT HABCAB(CO)NH
  • (4,3)D GFT HCCH aliphatic
  • (4,3)D GFT HCCH-COSY aromatic
  • 3D HNCO
  • 3D HN(CA)CO
  • 3D CBCA(CO)NH
  • 3D HNCACB
  • 3D HBHA(CO)NH
  • (H)CCH-COSY aliphatic
  • (H)CCH-COSY aromatic
  • 2D [13C,1H]-CT-HSQC, 28ms delay - optimized for methyl groups
  • 2D [13C,1H]-CT-HSQC, 56ms delay (optional)
  • 2D [13C,1H]-CT-HSQC, 42ms delay (optional)
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional - can also be run for the NC sample)

Arrowsmith group

Data acquisition is taylored for ABACUS approach for resonance assignment. Most 3D spectra are collected with Non-uniform sampling and processed with Multidimensional decomposition,


NH-rooted  CH-rooted (aliphatic) CH-rooted (aromatic) Other
  • 2D [15N,1H]-HSQC
  • 3D 15N-NOESY-HSQC
  • 3D HNCO
  • 3D HNCA
  • 3D CBCA(CO)NH
  • 3D HBHA(CO)NH
  • 3D C(CCO)NH-TOCSY (optional)
  • 3D H(CCCO)NH-TOCSY (optional)
  • 2D [13C,1H]-HSQC
  • 2D [13C,1H]-CT-HSQC
  • 3D 13C-NOESY-HSQC
  • 3D (H)CCH-TOCSY
  • 3D H(C)CH-TOCSY
  • 2D [13C,1H]-HSQC
  • 2D [13C,1H]-CT-HSQC (optional)
  • 3D 13C-NOESY-HSQC
  • 3D (H)CCH-TOCSY (optional)
  • 3D H(C)CH-TOCSY (optional)
  • 1D presaturation
  • 1D 15N T1
  • 1D 15N T2
  • 2D long-range [15N,1H]-HSQC or HMQC for histidine rings (optional)
  • 2D HE(CECDCG)CB (optional)
  • 2D HD(CDCG)CB (optional)
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